Get Synthetic Peptides as Antigents PDF
By M.H.V. Van Regenmortel and S. Muller (Eds.)
ISBN-10: 008085897X
ISBN-13: 9780080858975
ISBN-10: 0444821759
ISBN-13: 9780444821751
This most recent version to the Laboratory thoughts sequence provides present cutting-edge use of artificial peptides in molecular biology and functional protocols on tips to conjugate peptides, immunize animals with peptides and computer screen immune responses to peptides in vitro. It supplies heritage details on antigenic specificity, prediction of antigenic websites in proteins and functions of peptides in immunology and virology, as probes in prognosis and as vaccines. The booklet additionally describes antigenicity of proteins and strategies to localize antigenic websites in addition to equipment for predicting epitoxes, and provides targeted protocols for peptide-carrier conjugation, immunization with peptides, and peptide immunoassays. the amount additionally describes usual use of antipeptide antibodies in molecular and mobile biology in addition to using peptides within the prognosis of viral infections and autoimmune illnesses, and using peptides as capability artificial vaccines. a very good version to a good sequence, to be had in hardbound and paperback.
Read or Download Synthetic Peptides as Antigents PDF
Similar chemistry books
B. Markert's Trace Elements- Their Distribution and Effects in the PDF
This quantity discusses significant components of fundamental quandary for the knowledge of the complexity linked to ecological hint point study. those comprise assets and fates of hint parts; analytical suggestions; and the distribution of hint components in biota and soil and sediment reservoirs. Case reports, box paintings and laboratory reviews intensively mentioned during this quantity are valuable to augment our wisdom approximately methods on the topic of the organic reaction of hint steel rigidity lower than lifelike environmental stipulations.
Catalysis performs an more and more serious function in glossy petroleum refining and easy petrochemical industries as industry calls for for and standards of petroleum and petrochemical items are regularly altering. As we input the twenty first century, new demanding situations for catalysis technology and expertise are expected in virtually each box.
Download PDF by Albin F. Turbak (Eds.): Synthetic Membranes:. Volume I Desalination
Content material: v. 1. Desalination -- v. 2. Hyper- and ultrafiltration makes use of. v. 1. The Loeb-Sourirajan membrane : the way it took place / Sidney Loeb -- opposite osmosis : a brand new box of utilized chemistry and chemical engineering / S. Sourirajan -- program of artificial membranes in water provide structures in Israel / P.
- A Textbook of Inorganic Chemistry
- Trace Element Analysis in Biological Specimens
- Chemistry: The Central Science (13th Edition)
- Handbuch des kathodischen Korrosionsschutzes: Theorie und Praxis der elektrochemischen Schutzverfahren, 4. Auflage
- Dendrimer Chemistry: Concepts, Syntheses, Properties, Applications
- Advanced Dairy Chemistry Volume 2 Lipids
Extra resources for Synthetic Peptides as Antigents
Example text
The significance of the inhibition data is hard to assess, as no indication was given of total radioactive counts of antigen used in the experiments. If, for instance, only a small percentage of antigen added was precipitated, the inhibition of this precipitation would be difficult to interpret in quantitative terms. , 1982). , 1980). It was found that the synthetic peptides corresponding to residues 22-55 and 72-89 of myoglobin reacted with antibovine myoglobin antibodies and thus also contained epitopes (Leach, 1983).
A positive cross-reaction is taken as evidence that the peptide approximates to an epitope of the protein. , 1983) that immunization with peptides leads to a high frequency of induction of antibodies capable of recognizing the native protein, this is no longer believed to be true. , 1988; Jemmerson, 1987; Kennel, 1982; Soderquist and Walton, 1980; Spangler, 1991). In order to demonstrate that antipeptide antibodies are capable of reacting with a truly native protein, the test should Ch. 1 MOLECULAR DISSECTION OF PROTEIN ANTIGENS AND THE PREDICTIONOF EPITOPES 37 be performed by a liquid-phase immunoassay that does not alter the conformation of the antigen (Spangler, 1991).
1 Methods used for localizing epitopes in proteins Method No. of residues Criterion for residue in epitope Crystallographic analysis 15-22 of antigen-antibody complexes Synthetic peptides as anti- 5-8 genic probes in binding studies Free peptides Peptides adsorbed to solid phase Peptides conjugated to a carrier Peptides attached to support used for synthesis 3-5 Identification of critical re5idues in peptides by 5ystematic replacement of rc\idue\ 5-8 Peptides sequence\ imerted i n recombinant protein5 Binding of antipeptide 5-8 antibodies to protein Binding of antiprotein 3-5 antibodies to chemically modified or mutated proteins Protection of antibody5-10 bound residues of epitope to chemical attack Competitive topographic Only relative mapping position of epitope is defned allocation Contact in epitope paratope interface Cross-reactive binding with antiprotein antibodies Decrease in cross-reactive binding Cross-reactive binding with antiprotein antibodies Induction of cross-reactive antibodies Decrease in binding compared to unmodified protein Chemical reactivity of individual residues Not applicable Ch.
Synthetic Peptides as Antigents by M.H.V. Van Regenmortel and S. Muller (Eds.)
by Christopher
4.4